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Catecholamines may be released in the myocardium in response to ischemia or adrenergic nerve stimulation. A metabolic change in the heart, which can be mimicked experimentally in myocytes and platelets, is stimulation of the neutral cytoplasmic cholinesterase (NCE). In the present study we have investigated whether NCE is the source of a heat-stable chemical mediator that has chemotactic activity, the authentic neutrophil chemotaxin (NCT), on activated human neutrophils (PMN). Purified NCE from heart and liver, isocarbacyclin, a stable analog of NCT, and acetylcholine are all active chemoattractants (half-maximal response at 1.4, 0.1, and 0.2 microM, respectively) on human neutrophils stimulated to chemotaxis by formyl-methionyl-leucyl-phenylalanine. These agents (at 1 microM) also blocked the chemotactic response to NCT (half-maximal inhibition at 1, 1.5, and 1.6 microM, respectively). Isocarbacycl
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